WebJan 10, 2024 · Main Text. Mammalian respiratory complex I (NADH:ubiquinone oxidoreductase or CI) is a large enzyme built from 44 different structural proteins. The definition of its assembly pathway faced several unique challenges: it is a huge enzyme (≈1 million Daltons); it combines very hydrophilic and very hydrophobic domains; being … WebCI is built in a stepwise fashion through the actions of several assembly factors. We employed interaction proteomics to interrogate the molecular associations of 15 core subunits and assembly factors previously linked to human CI deficiency, resulting in a network of 101 proteins and 335 interactions (edges).
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Dynamics of Human Mitochondrial Complex I Assembly
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