Flag antibody proteintech
WebApr 10, 2024 · The extracts were mixed with 5 μg of an anti-FLAG antibody (Proteintech) or a normal IgG antibody (Millipore) and incubated overnight at 4 °C with end-over-end rotation. The next day, the RNP ... WebMouse Anti Flag Monoclonal M2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - …
Flag antibody proteintech
Did you know?
WebAug 3, 2024 · The signals were detected with anti-GFP and -FLAG antibodies (Proteintech). Cell-free degradation assay. Two-week-old seedlings of wild type (DJ) or sapk9 mutant were used to extract protein. Total protein was extracted in the degradation buffer (25 mM Tris-HCl, pH 7.5, 10 mM NaCl, 10 mM MgCl 2, 5 mM DTT, and 10 mM … WebFLAG-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The FLAG-tagged protein binds to the FLAG-tag specific monoclonal antibody conjugated on an agarose gel. After washing away residual impurities, bound FLAG-tag proteins can be eluted off the affinity column by high concentration of the FLAG-tag …
WebFLAG ® Tag Protein Purification. We offer a variety of agarose affinity purification gels, magnetic beads, purification kits, and coated plates for isolation and purification of FLAG … WebThe proteins retained on beads were subjected to immunoblot using anti-GST and anti-Flag antibodies. F 293T cells were transfected with si-Control or si-p62 under MG132 treatment. ... (1:5000; SA00001-1), and goat polyclonal anti-rabbit IgG (1:5000; SA00001-2) were obtained from Proteintech; antibody against pan–acetyl-lysine (1:2000, 9441S ...
WebFor in vitro co-immunoprecipitation (co-IP) analysis, FLAG-tagged proteins were produced in HTR-8/Svneo and JAR cells transfected with LV-PADI6. Total cell lysates were incubated overnight at 4°C with anti-YAP1 antibodies (Proteintech, Wuhan, China), anti-FLAG antibodies (Sigma, MO, USA), or normal IgG (Beyotime, Shanghai, China) as a control. WebFeb 2, 2024 · Flag IP was performed and analyzed by western blotting with Flag or myc antibodies. (C) N-TAD domain of AR interacted with SMAD3. Myc-SMAD3 was co-expressed with the Flag-tagged AR fragments (N-terminal transactivation domain, N-TAD; DNA binding domain, DBD; Ligand-binding domain, LBD) in 293T cells, and analyzed as …
WebApr 6, 2024 · Cells were then incubated with primary antibody for 2 h at RT, followed by incubation with secondary antibody for 1 h. A wash step with 0.1% PBS-0.1%Tween (pH = 7.5) was applied after each incubation step. Finally, the cells were imaged under a confocal microscope. Proteomics. Flag bead-tagged proteins were loaded and run on …
WebMouse Anti Flag Monoclonal M2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more solar powered phone charger best buyWebReal Time Stock Check. ITEM #: 8158795. Anti-FLAG TAG (Polyclonal)^ Anti-FLAG TAG (Polyclonal) Antibody, Host Species: Rabbit, Cross Reactivity: Recombinant protein, … solar powered personal fanWebProteintech Anti-DDDDK Tag Polyclonal, Catalog # 20543-1-AP. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. ... adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are ... solar powered pet heaterWebFLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been … sly and the family stone family affair songWebJun 15, 2012 · Abstract. The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several … solar powered pergola lightsWebDYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N … sly and the family stone fresh rarWebApr 8, 2024 · The antibodies used in IHC analysis are listed in Supplementary Table S4. ... E. Coli derived GST-Vector or GST-PRDX1 fusion protein was purified and prepared by Proteintech (Wuhan, China). Briefly, the total protein of 293T cells with or without Flag-tagged IRAK1 overexpression was incubated with GST-PRDX1 or GST-Vector fusion … solar powered phone charger nz