Pmcherry-gfp-lc3
WebmCherry-GFP-LC3 Use Lentiviral Tags Expression Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pDEST-CMV mCherry-GFP-LC3B WT Plasmid #123230 Purpose Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter. Depositor WebFeb 19, 2024 · To perform this assay, cultured cells with stable expression of mCherry-GFP-LC3 are treated with an experimental stimulus and then harvested for flow cytometry. The necessary controls include cells treated …
Pmcherry-gfp-lc3
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WebpmCherry-EGFP-LC3b. Catalog No. PVT10398 Packing 2ug . pmCherry-EGFP-LC3b Information Function ORF expression plasmid. Promoter: CMV. Replicator: pUC ori, F1 ori, … WebPlasmid pCDH-EF1a-mCherry-EGFP-LC3B from Dr. Sang-Hun Lee's lab contains the insert mCherry-GFP-LC3 and is published in Sci Adv. 2024 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2024 Feb. This plasmid is available through Addgene.
WebDec 27, 2024 · LC3（light chain 3）简称于MAP1LC3 (microtubule-associated proteins light chain 3)，是目前公认的自噬标记物。哺乳动物中的LC3与酵母中的自噬相关蛋 … WebSep 17, 2024 · In response to H. pylori infection, the levels of LC3-II were strongly increased in both cell lines (Fig. 2b) and correlated with an increase in the autophagosome number in AGS-LC3 cells expressing the tandem-tagged mCherry-GFP-LC3 protein (Fig. 2c, d). All these results indicate that H. pylori infection induced autophagy in GC cell lines.
WebJan 12, 2024 · Autophagy Formation Traced by mRFP-GFP-LC3 Double Fluorescence Assay. Chondrocytes were cultured on coverslips for 24 h and then transfected with pmCherry-GFP-LC3 plasmid utilizing Lipofectamine 2000 reagent (Invitrogen) for 24 h. Next, the cells were fixed with 4% paraformaldehyde and observed with a confocal fluorescence … WebJun 2, 2011 · When fused to the autophagic marker LC3, GFP fluorescence is quenched upon fusion between autophagosome and lysosome due to the low lysosomal pH, while mCherry remains fluorescent until degradation by lysosomal proteases [ 18, 24 ]. Cells were stably transfected with tandem-LC3.
Webtandem mCherry-GFP-LC3 construct in neurons through the nervous system and demonstrated efficient and accurate measurements of LC3 flux after pharmacological …
WebDec 17, 2024 · Leica TCS-SPE DMI4000B (Leica Microsystems, Wetzlar, Germany) was used for obtaining confocal images of HEK293 cells expressing Cherry-GFP-LC3. 2.7. Statistical analysis Statistical analyses were conducted by Student’s t -test. A P -value of less than 0.05 was considered statistically significant. 3. Results 3.1. emily lindholmWebDec 1, 2024 · Western blot, mCherry-GFP-LC3 labeling, immunofluorescence, and RNA-seq were performed to examine the regulatory mechanism of SCV-2-S in autophagic … drag me down one direction in g majorWebMost ATG factors but not LC3 homologs detach from the isolation membranes before completion of autophagosomes. These isolation membrane markers are localized on the … emily linaresemily lind datingWebJul 22, 2024 · Using a reporter construct expressing a fusion protein of GFP, LC3, RFP, and a non-cleavable variant of LC3B, LC3BΔG, this system allows, similarly to the double-labeled LC3B, monitoring of ... emily lind 2022WebSep 13, 2009 · To generate MDCK cells stably expressing GFP–LC3 and p62-3×Myc, ... MDCK/pmCherry–LC3 cells were seeded at 4 × 10 4 cells ml −1 on 35-mm glass bottom dishes and cultured for 4 days at 37 °C. drag me out blind and blurred lyricsWebOct 7, 2014 · Not all optineurin-labeled mitochondria are LC3-positive at this time point (yellow arrows). (C) Increased recruitment of LC3 to optineurin-labeled mitochondria after 1.5 h of CCCP treatment (white arrows). (D–F) HeLa cells expressing parkin, GFP-LC3, and BFP-mito (pseudocolored red). Formation of LC3 autophagosomes around damaged ... drag me down remix